Annual Review of Immunology Volume 11 1993 by Annual Reviews

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Annu. Rev. Immunol. 7:445-80 34. Schell, S. , Fitch, F. W. 1989. Pretreatment of cloned helper T lymphocytes with interleukin 2 induces unresponsiveness to antigen and concanavalin A, associated with decreased inositol phosphate and diacylglyccrol production. J. lmmunol. 143:1499-505 35. , l~loldwin, R. , Dunn, D. , Nau, G. , Fitch, F. W. 1986. PMAalone induces proliferation of some murine T cell clones but not others. J. Immunol. 137:2755-60 36. Gajewski, T. , Schell, S. , Fitch, F. W. 1989. Regulation of T cell activation: Differences amongT cell subsets.

Glasebrook, 17. Mosmann,T. , Schumasher, J. L. FrequenciesofT cells secretStreet, N. , ing IL-2 and/or IL-4 amongunprimed Fong, T. A. , Bond, M. , Moore, CD4+populations. Evidence that K. , Fiorentino, D. F. Diversity ofcytokinesynthesis and to clones whichsecrete onlyIL-4. Immufunction of mouseCD4+ T cells. Immunol. Lett. 23:227-34 nol. Rev. 123:209-29 8. , Hauser, C. 18. Fiorentino, D. , Vieira, 1992. , Mosmann, T. , T cells producingIL-2 or IL-4. J. ImmuMoore, K. , O’Garra, A. 1991. ILnol.

Murinealloreactive clones usually have been obtained from spleen cells. Most humanT cell clones have been derived from peripheral blood lymphocytes. The APC studied have been from relatively few sources. Spleen cells have been most commonlyused to stimulate murine T cell clones. The anatomical location of lymphocytesis determined in large part by the expression of cell surface molecules involved in homingto various lymphoidtissues (52). It is not yet clear these homingreceptors are differentially expressed on T cell subsets or whether functional characteristics of T lymphocytesare influenced by their anatomic location.

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